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hydrocortisone  (PromoCell)


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    PromoCell hydrocortisone
    Hydrocortisone, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+growth+medium+2+kit/Endothelial+Cell+Growth+Medium+MV+2+KIT/pmc13019081-439-15-16
    Average 96 stars, based on 123 article reviews
    hydrocortisone - by Bioz Stars, 2026-10
    96/100 stars

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    Cell Culture:

    Article Title: VAP-1-Mediated M2 Macrophage Infiltration Underlies IL-1?- but Not VEGF-A-Induced Lymph- and Angiogenesis
    Article Snippet: After an overnight incubation, sections were washed and stained for 20 minutes with Alexa Fluor488 goat anti-rabbit IgG (10 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A11034","term_id":"489250","term_text":"A11034"}} A11034 ; Invitrogen), Alexa Fluor488 donkey anti-goat IgG (10 μg/mL, A-11055; Invitrogen), and Alexa Fluor647 goat anti-rabbit IgG (10 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A21244","term_id":"641366","term_text":"A21244"}} A21244 ; Invitrogen). .. Human umbilical vein endothelial cells (HUVECs) (C-12200; PromoCell, Heidelberg, Germany) or human lymphatic endothelial cells (HLECs) (2500; ScienCell Research Laboratories, Carlsbad, CA) were routinely cultured in endothelial cell growth medium-2 KIT (C-22111; PromoCell) or supplemented with 2% fetal bovine serum (FBS) endothelial cell medium (1001; ScienCell), respectively, in a humidified incubator under 5% CO 2 at 37°C. .. At day 3 after 30 ng IL-1β implantation, conjunctiva and cornea were harvested and lysed in a mammalian cell lysis kit (MCL1; Sigma Chemical Co.).

    Article Title: Blood vessel endothelial VEGFR-2 delays lymphangiogenesis: an endogenous trapping mechanism links lymph- and angiogenesis
    Article Snippet: After an overnight incubation, sections were washed and stained for 20 minutes with goat α-rat IgG CY5 conjugate (10 μg/mL, 81-9516; Zymed Labo), Alexa Fluor488 donkey α-goat IgG (10 μg/mL, A-11 055; Invitrogen), and Alexa Fluor647 goat α-rabbit IgG (20 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A21244","term_id":"641366","term_text":"A21244"}} A21244 ; Invitrogen). .. Cell culture Human umbilical vein endothelial cells (HUVECs; PromoCell; C-12 200 or Cambrex) were routinely cultured in endothelial cell growth medium-2 KIT (PromoCell; C-22111) supplemented with 2% fetal bovine serum (FBS) in a humidified incubator under 5% CO 2 at 37°C. ..

    Article Title: Positive Feedback Defines the Timing, Magnitude, and Robustness of Angiogenesis
    Article Snippet: Pooled primary Human umbilical vein endothelial cells (HUVECs) were purchased from Promocell (C-12203). .. Cells were cultured in endothelial cell basal medium supplemented with endothelial cell growth medium 2 kit (Promocell; C-22111) at 5% CO2 and a temperature of 37°C. ..

    Article Title: VAP-1-Mediated M2 Macrophage Infiltration Underlies IL-1?- but Not VEGF-A-Induced Lymph- and Angiogenesis
    Article Snippet: After an overnight incubation, sections were washed and stained for 20 minutes with Alexa Fluor488 goat anti-rabbit IgG (10 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A11034","term_id":"489250","term_text":"A11034"}} A11034 ; Invitrogen), Alexa Fluor488 donkey anti-goat IgG (10 μg/mL, A-11055; Invitrogen), and Alexa Fluor647 goat anti-rabbit IgG (10 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A21244","term_id":"641366","term_text":"A21244"}} A21244 ; Invitrogen). .. Cell Culture Human umbilical vein endothelial cells (HUVECs) (C-12200; PromoCell, Heidelberg, Germany) or human lymphatic endothelial cells (HLECs) (2500; ScienCell Research Laboratories, Carlsbad, CA) were routinely cultured in endothelial cell growth medium-2 KIT (C-22111; PromoCell) or supplemented with 2% fetal bovine serum (FBS) endothelial cell medium (1001; ScienCell), respectively, in a humidified incubator under 5% CO 2 at 37°C. .. Western Blot Analysis At day 3 after 30 ng IL-1β implantation, conjunctiva and cornea were harvested and lysed in a mammalian cell lysis kit (MCL1; Sigma Chemical Co.).

    Article Title: Endothelial cell source dictates the expression and release of fibrinolytic markers in a proinflammatory environment
    Article Snippet: EA.hy926 cells (ATCC), a hybridoma cell line derived from HUVECs and A549 epithelial cells, were cultured in Dulbecco’s modified eagle medium (DMEM; Gibco) supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (Gibco). .. Commercially obtained pooled HUVECs and single donor HCAECs were purchased and cultured in endothelial cell growth medium-2 kit (EGM-2; Promocell GmbH) and used experimentally between passages 2 – 5 [8, 29]. .. Cell viability was determined, using ReadyProbesTM Cell Viability Imaging Kit, Blue/Green (ThermoFisher).

    Article Title: Phototoxicity of Vascular Endothelial Cells Caused by Contact with Talaporfin Sodium for 15-120 Min: In Vitro and In Vivo Studies.
    Article Snippet: Objective: To reveal the mechanism of vascular patency in the myocardium after photosensitization immediately after talaporfin sodium (TS) injection in a canine model, we investigated acute injury to vascular endothelial cells (VECs) in vitro and in vivo.. Background data: There are many reports of vascular shutdown within the target region in photodynamic therapy with TS.. Vascular patency within healthy canine myocardium in which a photosensitization reaction starts immediately after injection of TS has been reported.

    Article Title: Blood vessel endothelial VEGFR-2 delays lymphangiogenesis: an endogenous trapping mechanism links lymph- and angiogenesis
    Article Snippet: After an overnight incubation, sections were washed and stained for 20 minutes with goat α-rat IgG CY5 conjugate (10 μg/mL, 81-9516; Zymed Labo), Alexa Fluor488 donkey α-goat IgG (10 μg/mL, A-11 055; Invitrogen), and Alexa Fluor647 goat α-rabbit IgG (20 μg/mL, {"type":"entrez-nucleotide","attrs":{"text":"A21244","term_id":"641366","term_text":"A21244"}} A21244 ; Invitrogen). .. Human umbilical vein endothelial cells (HUVECs; PromoCell; C-12 200 or Cambrex) were routinely cultured in endothelial cell growth medium-2 KIT (PromoCell; C-22111) supplemented with 2% fetal bovine serum (FBS) in a humidified incubator under 5% CO 2 at 37°C. ..



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